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- W2386416135 abstract "Objective To investigate the relationship between HBV DNA determined by fluorescence quantitative polymerase chain reaction assay(FQ PCR) and HBV serum markers.Methods Serum samples whose HBV DNA had been determined by FQ PCR were assayed again by ELISA for HBV PreS1 antigen (PreS1)and other HBV markers.Results Of 186 samples positive for HBV DNA,184 (98.92%) was detected positive for HBsAg,175(94.09%) positive for HBcAb,two samples were negative for HBsAg,one of which was positive for HBcAb only.PreS1 and HBeAg was detected in 115(61.83%)and 124(66.67%) samples respectively.Both PreS1 and HBeAg were detected in 79 samples(42.47%),and samples positive for either of them was 160(86.02%).There was no statistical difference between positive rate of PreS1 and HBeAg.Out of 114 samples negative for HBV DNA but positive for HBsAg,HBcAb was detected in 110(96.49%) samples,while PreS1 and HBeAg was detected in 26(22.81%)and 6(5.26%) samples respectively,both were detected in 4 samples( 3.51 %).There was a statistical difference between positive rate of PreS1 and HBeAg in these samples( P 0.001).There was no statistical difference for PreS1 and HBeAg positive rate between samples of high and low HBV DNA levels.For samples positive for HBV DNA,the HBV DNA levels(denary logarithm value)of samples positive for only PreS1 or HBeAg were 6.654±2.203 ( x±s ) and 6.892 ± 1.579 respectively,and HBV DNA level was 6.706±1.643 and 6.321±1.763 for samples positive and negative for both respectively,and there was no statistical difference between either two groups.Conclusion PreS1 and HBeAg correlate strongly with HBV DNA and may be used as markers of HBV replication,and better effect will be achieved if they can be applied together.There was no evident relationship between their existent patterns and HBV DNA levels in serum.Although there was no correlation between HBcAb and HBV replication,HBV DNA should be determined for samples with HBcAb as an unique marker,so that occult HBV infection could be detected." @default.
- W2386416135 created "2016-06-24" @default.
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- W2386416135 date "2004-01-01" @default.
- W2386416135 modified "2023-09-28" @default.
- W2386416135 title "Relationship between HBV DNA determined by FQ-PCR and HBV serum markers" @default.
- W2386416135 hasPublicationYear "2004" @default.
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