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- W2386420461 abstract "[Objective] Specific growth rate of Escherichia coli could be significantly decreased by L-alanine, which would result in reduction in L-alanine volumetric productivity. Therefore, the λpR-pLpromoter was used as a thermo-controllable genetic switch to coordinate the processes of cell growth and L-alanine production in E. coli. [Methods] Synthetic routes for acetate, formate, ethanol, succinate and lactate as well as for L-alanine recemization were inactivated by deleting the corresponding genes(ack A-pta, pfl B, adh E, frd A, ldh A, dad X) in the wild-type E. coli B0016 to generate B0016-060 B. Subsequently, alanine dehydrogenase derived from Geobacillus stearothermophilus was cloned downstream of the pL promoter and expressed in B0016-060 B to produce B0016-060B/p PL-ala D. Shake-flask and bioreactor experiments were conducted to investigate the properties of cell growth and L-alanine fermentation. [Results] Deletions of the competing pathways significantly decreased by-products accumulation with formation of low levels of acetate, succinate and ethanol. Strain B0016-060B/p PL-ala D hardly produced L-alanine during cell growth phase at 28 °C, which facilitated high growth rate. Meanwhile, efficient L-alanine production was obtained when cultured at 42 °C under oxygen-limited conditions. In bioreactor experiment, strain B0016-060B/p PL-ala D produced 67.2 g/L L-alanine, with a productivity of 2.06 g/(L·h). [Conclusion] Efficient cell growth and L-alanine production were realized simply by switching the cultivation temperature." @default.
- W2386420461 created "2016-06-24" @default.
- W2386420461 creator A5028404144 @default.
- W2386420461 date "2015-01-01" @default.
- W2386420461 modified "2023-09-23" @default.
- W2386420461 title "L-alanine production in recombinant Escherichia coli with thermo-regulated genetic switch" @default.
- W2386420461 hasPublicationYear "2015" @default.
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