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- W2386435331 abstract "Objective Liver fibrosis is characterized by increased deposition and altered composition of extracellular matrix. The aim of this study was to construct recombinant vector carrying interstitial collagenase(MMP-13) and investigate if the expression of collagen I could be inhibited in rat hepatic stellate cell(HSC) overexpressing MMP-13 in vitro. Methods The plasmid expressing MMP-13(dl6-95-MMP-13) was constructed through inserting the full length of MMP-13 gene into the AAV vector dl6-95 which includes the ITR of AAV. Then,dl6-95-MMP-13 or dl6-95 plasmid was transfected into HSC for 7 days. The level of MMP-13 and collagen I mRNAs and proteins were analyzed by real time PCR and Western blotting. Results The plasmid expressing MMP-13(dl6-95-MMP-13) was constructed successfully. After dl6-95-MMP-13 plasmid was transfected into HSC for 7 days,real time PCR results showed that the level of MMP-13 mRNA increased significantly compared with HSC transfected with dl6-95 plasmid(without MMP-13),whereas the transcription of collagen I had only slight increase. Western blotting results showed that both the pro-MMP-13 and active MMP-13 increased significantly,especially the active form of MMP-13. After transfected with dl6-95-MMP-13,the expression of collagen I was inhibited significantly. Conclusion MMP-13 overexpression in HSC could inhibit the expression of collagen I significantly. This inhibition occurred mainly through degradation of collagen I protein,rather than through regulating the transcription of collagen I." @default.
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- W2386435331 date "2009-01-01" @default.
- W2386435331 modified "2023-09-23" @default.
- W2386435331 title "Overexpression of MMP-13 Inhibited Collagen I Expression in Rat Hepatic Stellate Cells" @default.
- W2386435331 hasPublicationYear "2009" @default.
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