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• W2386478579 abstract "Objectives To evaluate in vitro and vivo osteogenetic effectiveness of hyaluronic acid mixed with adenovirus mediated human bone morphogenetic protein 2 gene (Adv hBMP 2) transferred bone marrow derived mesenchymal stem cells (BMSCs) of rabbit.Methods In the in vitro test,the BMSCs were collected from bone marrow of rabbits and cultured in vitro.There were 4 groups in the test:Ⅰ.Adv hBMP 2 transfected BMSCs+HA;Ⅱ.Adv hBMP 2 transfected BMSCs;Ⅲ.Untransfected BMSCs+HA:Ⅳ.Untransfected BMSCs.These BMSCs were evaluated by immunoprecipitation (IP)/Western blotting,RT PCR,ALP activity assay,and von Kossa staining.In in vivo test,12 node mice (24 sides) subject to the injection of the cultured cells into the muscle were divided into four groups:Ⅰ.Adv hBMP 2 transfected BMSCs+HA ( n =8);Ⅱ.Adv hBMP 2 transfected BMSCs ( n =8);Ⅲ.Untransfected BMSCs ( n =4);Ⅳ.HA ( n =4).The osteoinductivity in muscle of nude mice was investigated by X ray examination,osteogenesis quantification and histological examination.Results Results of in vitro test:hBMP 2 and bone sialoprotein mRNA were expressed,and the high ALP activities and formation of calcified nodes were detected in the groups of Adv hBMP 2 transfected BMSCs+HA and Adv hBMP 2 transfected BMSCs,both of which were more significant than those in the groups of untransfected BMSCs+HA and untransfected BMSCs;Results of in vivo test:Under X ray examination the ectopic bones in the muscles of nude mice were observed in the groups of Adv hBMP 2 transfected BMSCs+HA and Adv hBMP 2 transfected BMSCs,and the amount of ectopic bones in the groups of Adv hBMP 2 transfected BMSCs+HA Adv hBMP 2 transfected BMSCs was (155.0±11.5) mg and (83.4±13.5) mg respectively,with the difference being statistically significant ( P 0.01).Histological examination revealed that there were chondrogenesis and osteogenesis in the groups of Adv hBMP 2 transfected BMSCs+HA Adv hBMP 2 transfected BMSCs and it seemed that chondrogenesis in the former was more than that in the latter.There was no ectopic bone formation in the groups of untransfected BMSCs and HA and the main component was fibrous and fat tissue histologically.Conclusion HA mixed with Adv hBMP 2 transfected BMSCs has good osteogenetic effects in vitro and osteogenetic and chondrogenetic effects in the muscle of nude mice.It is a potential injectable method to repair the osteochondral defects in the animal models,possibly in the human beings." @default.
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• W2386478579 date "2004-01-01" @default.
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• W2386478579 title "In vitro and in vivo osteogenetic effects of hyaluronic acid mixed with BMP-2 gene transfected bone marrow derived mesenchymal stem cells" @default.
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