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- W2386482781 abstract "In order to analyze the characteristic of the alpacas KIT gene structure and the biological activity of the expression product,KIT gene of the alpacas was amplified by RT-PCR from alpaca's skin tissue RNA.Sequence analysis revealed that the fragments of exon10-14 of KIT gene of alpacas were 414 bp,It includes the partial sequence of exon10(30 bp),exon11(127 bp),exon12(125 bp),exon13(91 bp),exon14(41 bp).Sequence nucleotide and deduced amino acid sequences were compared with homologous sequences from cattle,goat,pig,humans and horses;it displayed a fairly high degree of conservation.The homologue of the nucleotide sequences are respectively 94 %,93 %,93 %,92 %,92 %;the homologue of the amino acid sequence were above 97 %.And the deduced polypeptide was got by the translation software and it was found that it coded 138 amino acids.And an amino acid substitution was detected in the polypeptide coded by exon10-14 of KIT gene in alpacas,which were V→I at the position of 2.The cDNA fragment was inserted into expression vector pET-32a(+) and the resulted plasmid was expressed in DH5α by IPTG induction.The results of SDS-PAGE analysis indicated that fusion protein was specifically expressed in the DH5α.The KIT protein,about 36 kD,is fusible.The recombinant protein was expressed highly,which was nearly 20 % of the total products." @default.
- W2386482781 created "2016-06-24" @default.
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- W2386482781 date "2008-01-01" @default.
- W2386482781 modified "2023-09-24" @default.
- W2386482781 title "Molecular Cloning and Procaryote Expression of KIT Gene Controlling Dominant White Coat Color in Alpacas(Lama pacos)" @default.
- W2386482781 hasPublicationYear "2008" @default.
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