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- W2386535648 abstract "The results of bisulfite sequencing and methylation-specific PCR showed that the promoter of AcMNPV immediate-early gene(ie0p)is CpG methylated at a relatively high level,and its methylation level changed during infection reaching the lowest around 8hpost infection.To study the effect of DNA methylation on the regulation of ie0 and ie1 promoters,plasmids p402-ie1and p402-ie0M-A,which can express IE0and IE1protein,respectively,and reporter plasmids pie0p-luc and pie1p-luc were reconstructed.Methylated reporter plasmid DNA was obtained by treating the plasmid DNA with bacterial methyltransferase M.SssⅠin vitro.The results verified that IE0activated ie0p,whereas IE1inhibited it.DNA methylation was found to reduce the activity of ie0 and ie1 promoter greatly.By contrast,both IE0and IE1increased ie1p activity,and maintained such activation after methylation.By cotransfecting different ratio of p402-ie1and p402-ie0M-A with reporter plasmid into Sf9cells,we demonstrated that DNA methylation could reverse the regulation by IE0and IE1on ie0p when IE0IE1.These results suggest that DNA methylation can affect baculovirus gene expression and regulation." @default.
- W2386535648 created "2016-06-24" @default.
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- W2386535648 date "2013-01-01" @default.
- W2386535648 modified "2023-09-23" @default.
- W2386535648 title "The Effect of DNA Methylation in AcMNPV ie0/ie1 Promoter on IE0/IE1 Novel Regulation" @default.
- W2386535648 hasPublicationYear "2013" @default.
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