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- W2386563260 abstract "The aim of this study was to establish a rapid multiplex PCR detection method for swine edema disease caused by Shiga toxin-producing Escherichia coli(STEC).Three pairs of specific primers based on Escherichia coli 16 SrDNA,Shiga toxin Stx2 esubunit A and fimbriae F18 ab subunit A were designed and the reaction conditions were optimized.At the same time,sensitivity and specificity of multiplex PCR were studied.The results showed that the amplification product sizes of positive control strain were 1 062,733 and 313bp.The specificity and sensitivity tests showed that there was no cross reaction with Salmonella enteritidis,Pasteurella multocida,Actinobacillus pleuropneumoniae,Haemophilus parasuis,Bordetella bronchiseptica and Streptococcus suis,as little as 1 875 CFU of bacteria could be detected by multiplex PCR.128 strains of pathogenic Escherichia coli isolated from swine were detected by multiplex PCR,36 strains of STEC were found,30 of them contained both Stx2 e and F18 abfimbriae genes,and the others only had Stx2 e gene.The results showed that the multiplex PCR could be used in rapid diagnosis and epidemiological investigation of swine edema disease." @default.
- W2386563260 created "2016-06-24" @default.
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- W2386563260 date "2015-01-01" @default.
- W2386563260 modified "2023-09-26" @default.
- W2386563260 title "Establishment and Application of Multiplex PCR to Identify Escherichia coli Causing Swine Edema Disease" @default.
- W2386563260 hasPublicationYear "2015" @default.
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