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- W2386572052 abstract "AIM: To observe the inhibitory effect of luteolin on recombinant human protein kinase CK2 holoenzyme and to confirm the inhibitory type by its enzyme kinetic analysis in vitro. METHODS: Recombinant human protein kinase CK2 α′ and β subunits were cloned, expressed and purified. The 2 subunits were mixed at equal molar ratio to reconstitute CK2 holoenzyme. Luteolin inhibitory effect was detected by determining the radio- activity of 32P of [γ- 32P] ATP incorporated into the CK2 substrate and CK2 kinetic analysis was carried out by using the Lineweaver-Burk plot. RESULTS: Luteolin was proved to inhibit strongly the holoenzyme activity of recombinant human protein kinase CK2 with IC_ 50 of 0.86 μmol/L. Kinetic studies of luteolin on recombinant human CK2 showed that luteolin acted as an inhibitor, competitive with ATP (K_i=0.19 μmol/L) and cooperative with casein (K_i=0.11 μmol/L). CONCLUSION: Luteolin is an effective inhibitor of protein kinase CK2 in vitro." @default.
- W2386572052 created "2016-06-24" @default.
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- W2386572052 date "2006-01-01" @default.
- W2386572052 modified "2023-09-24" @default.
- W2386572052 title "Inhibitory effect of luteolin on recom- binant human protein kinase CK2 holoenzyme in vitro" @default.
- W2386572052 hasPublicationYear "2006" @default.
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