Matches in SemOpenAlex for { <https://semopenalex.org/work/W2386574390> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2386574390 abstract "Objective To study the influence of TGF-β1 on proliferation and differentiation of HPDLFs in vitro.Methods HPDLFs were primarily cultured.The effect of TGF-β1 on the proliferative ability of HPDLFs was evaluated with MTT assay.The effects of TGF-β1 on the total amount of protein and the cell cycle were detected by coomassie brilliant blue(CBB) method and flow cytometry.The effects of TGF-β1 on the alkaline phosphatase(ALP) activity and expression of ALP mRNA were measured with the enzyme kinetic method and reverse-transcription polymerase chain reaction(RT-PCR).Results TGF-β1(2～ 10 μg/L)significantly promoted the proliferative ability of HPDLFs and the changes depended on dosage and time(P0.05).The total amount of protein was dramatically increased and the ALP activity was enhanced for 48 h with TGF-β1(2～ 10 μg/L),P0.05.After 48 h of TGF-β1(10 μg/L),the G1 phase cells reduced(P0.05),the S phase cells and proliferation index(PI) significanctly increased(P0.05).The expression of ALP mRNA was evidently up-regulated by different concentrations of TGF-β1(2～10 μg/L)after 48 h(P0.05).Conclusions These findings suggest that TGF-β1 was capable of producing an accelerative effect on the proliferation and differentiation functions on HPDLFs.It may be related to TGF-β1 enhancing cell DNA,protein synthesis,ALP mRNA expression and ALP activity." @default.
- W2386574390 created "2016-06-24" @default.
- W2386574390 creator A5012287238 @default.
- W2386574390 date "2008-01-01" @default.
- W2386574390 modified "2023-09-28" @default.
- W2386574390 title "Influence of TGF-β_1 on proliferation and differentiation of HPDLFs in vitro" @default.
- W2386574390 hasPublicationYear "2008" @default.
- W2386574390 type Work @default.
- W2386574390 sameAs 2386574390 @default.
- W2386574390 citedByCount "0" @default.
- W2386574390 crossrefType "journal-article" @default.
- W2386574390 hasAuthorship W2386574390A5012287238 @default.
- W2386574390 hasConcept C104317684 @default.
- W2386574390 hasConcept C105580179 @default.
- W2386574390 hasConcept C118131993 @default.
- W2386574390 hasConcept C153911025 @default.
- W2386574390 hasConcept C160160445 @default.
- W2386574390 hasConcept C181199279 @default.
- W2386574390 hasConcept C185592680 @default.
- W2386574390 hasConcept C202751555 @default.
- W2386574390 hasConcept C2910938049 @default.
- W2386574390 hasConcept C553184892 @default.
- W2386574390 hasConcept C55493867 @default.
- W2386574390 hasConcept C62112901 @default.
- W2386574390 hasConcept C86803240 @default.
- W2386574390 hasConcept C95444343 @default.
- W2386574390 hasConceptScore W2386574390C104317684 @default.
- W2386574390 hasConceptScore W2386574390C105580179 @default.
- W2386574390 hasConceptScore W2386574390C118131993 @default.
- W2386574390 hasConceptScore W2386574390C153911025 @default.
- W2386574390 hasConceptScore W2386574390C160160445 @default.
- W2386574390 hasConceptScore W2386574390C181199279 @default.
- W2386574390 hasConceptScore W2386574390C185592680 @default.
- W2386574390 hasConceptScore W2386574390C202751555 @default.
- W2386574390 hasConceptScore W2386574390C2910938049 @default.
- W2386574390 hasConceptScore W2386574390C553184892 @default.
- W2386574390 hasConceptScore W2386574390C55493867 @default.
- W2386574390 hasConceptScore W2386574390C62112901 @default.
- W2386574390 hasConceptScore W2386574390C86803240 @default.
- W2386574390 hasConceptScore W2386574390C95444343 @default.
- W2386574390 hasLocation W23865743901 @default.
- W2386574390 hasOpenAccess W2386574390 @default.
- W2386574390 hasPrimaryLocation W23865743901 @default.
- W2386574390 hasRelatedWork W2074576914 @default.
- W2386574390 hasRelatedWork W2106096051 @default.
- W2386574390 hasRelatedWork W2127420015 @default.
- W2386574390 hasRelatedWork W227836394 @default.
- W2386574390 hasRelatedWork W2354941014 @default.
- W2386574390 hasRelatedWork W2355245065 @default.
- W2386574390 hasRelatedWork W2360519037 @default.
- W2386574390 hasRelatedWork W2365464264 @default.
- W2386574390 hasRelatedWork W2373697793 @default.
- W2386574390 hasRelatedWork W2378770167 @default.
- W2386574390 hasRelatedWork W2384654391 @default.
- W2386574390 hasRelatedWork W2392727687 @default.
- W2386574390 hasRelatedWork W2399682307 @default.
- W2386574390 hasRelatedWork W2413268920 @default.
- W2386574390 hasRelatedWork W2529564640 @default.
- W2386574390 hasRelatedWork W2741715350 @default.
- W2386574390 hasRelatedWork W2990098276 @default.
- W2386574390 hasRelatedWork W3119755141 @default.
- W2386574390 hasRelatedWork W630449823 @default.
- W2386574390 hasRelatedWork W3152404756 @default.
- W2386574390 isParatext "false" @default.
- W2386574390 isRetracted "false" @default.
- W2386574390 magId "2386574390" @default.
- W2386574390 workType "article" @default.