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- W2386630752 abstract "The objective of this study was to determine the effect of flow cytometrically-sorted, stained and unsorted sperm on in vitro fertilization and blastocyst development of bovine oocytes A total of 4273 bovine oocytes and three sperm treatments (sorted, stained and unsorted) from 3 bulls (3 to 5 replicates of each bull) were examined Data were analyzed by ANOVA procedures Analysis revealed that there was no significant difference in blastocyst rate among the three types of sperm (204% vs 2262% and 2214% ); however, the cleavage rate of oocytes fertilized with sorted sperm (5366%) was lower(P005)than that of unsorted sperm (7193%) There were significant differences in cleavage or blastocyst rates among bulls tested H008 produced fewer(P005) embryos than H010(174% vs 251% )The results indicated that sorting procedure or stain did not affect embryo development to blastocyst, which demonstrated that flow cytometrically-sorted sperm can be used for efficient in vitro production The finding that fertilization and embryo development rates with sorted, stained unsorted sperm were variable among bulls indicates that choosing high quality sperm from bulls for sperm sorting can increase the efficient in vitro fertilization" @default.
- W2386630752 created "2016-06-24" @default.
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- W2386630752 date "2002-01-01" @default.
- W2386630752 modified "2023-09-24" @default.
- W2386630752 title "Effect of flow cytometrically-sorted sperm on in vitro fertilization of bovine oocytes" @default.
- W2386630752 hasPublicationYear "2002" @default.
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