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- W2386654609 abstract "The isolation and the function analysis of male-specific promoter is basic for heterosis and molecular breeding. A putative promoter Tsp2 was amplified and cloned from rice (Oryza sativa L.) genomic DNA by polymerase chain reaction (PCR) with the primers designed according to the sequence of rice anther-specific gene RA8. Tsp2 was cloned into pMD18-T vector and sequenced. Sequence analysis showed that it had 828 bp in length and contained TATA box and CAAT box. Tsp2 had 97% identity with RA8. Tsp2 was fused into reporter gene GFP and constructed to plant expression vector p1304-rap. The expression vector p1304-rap was transformed into anther of tobacco by Particle Bombardment. The transient expression of GFP was observed in tapetum particularly. No GFP expressed in leaf and pistil as control. The results in tobacco (Nicotiana tobacum) anther indicated the activities of the promoter. The Tsp2 promotor fragment was cloned from rice genome. However it could work well in tobacco. Therefore, the Tsp2 promoter should be used widely in heterosis and molecular breeding of all dicot and monocot crops. Fig 5, Ref 9" @default.
- W2386654609 created "2016-06-24" @default.
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- W2386654609 date "2008-01-01" @default.
- W2386654609 modified "2023-09-25" @default.
- W2386654609 title "Cloning and Activity Assaying of Anther-specific Promoter in Rice" @default.
- W2386654609 hasPublicationYear "2008" @default.
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