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- W2386673091 abstract "Objective:To investigate the effects of lipopolysaccharides(LPS) on the expression of IL-6 mRNA in human peripheral blood mononuclear cells. Methods:Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood treated with ethylenediaminetetraacetic acid (EDTA) and stimulated by LPS. Total RNA extracted from the PBMC was reversely transcripted to cDNA,which used as templates for hot-start PCR amplification of IL-6. Sandwich hybridization occurs between IL-6 amplicon,capture probe and detection probe. The hybridization signals were detected utilizing avidin-horseradish peroxidase and its substracts. Specific IL-6 primers and probes were designed using Primer Premier 5.0 software.Results:LPS is an effective inducer which can increase IL-6 mRNA. Expression of IL-6 mRNA is positively correlated with the concentration of LPS. Abundance of IL-6 mRNA varied with the time of LPS*0*8s stimulation and peaked at 4 h.Conclusion:LPS can enhance the expression of IL-6 mRNA in human PBMC." @default.
- W2386673091 created "2016-06-24" @default.
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- W2386673091 date "2004-01-01" @default.
- W2386673091 modified "2023-09-23" @default.
- W2386673091 title "Effects of lipopolysaccharides on the expression of IL-6 gene in human peripheral blood mononuclear cells" @default.
- W2386673091 hasPublicationYear "2004" @default.
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