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- W2386712640 abstract "Objective To study on the possibility of tissue engineering rat skin constructed with chitosan sponge. Methods The epidermal keratogenetic cells and dermal fibroblasts were formed by in vitro secondary cultured rat keratin. Then seeded them on the both side of ready made 3 dementional porous chitosan scafford respectively and the multilayered artificial skin was constructed by culture. The histological pattern and dynamic change of ultramicrostructure in the multilayered artificial skin were studied with microscope and electron microscope. Results The epidermal and dermal construction of the tissue engineering were seen under microscope. As the culture duration elongate the epiderm was ripe and proliferated and dermis was gradually formed. The matrix scafford was degraded and absorbed gradually and substituted by the extracellular matrix which was secreted by dermal cells. Conclusion It is demonstrated that the histological construction and metabolic function of the tissue engineering skin constructed by chistosan matrix scafford are quite similar to that of natural skin." @default.
- W2386712640 created "2016-06-24" @default.
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- W2386712640 date "2003-01-01" @default.
- W2386712640 modified "2023-09-25" @default.
- W2386712640 title "Tissue Engineering Rat Skin with Chitosan Sponge" @default.
- W2386712640 hasPublicationYear "2003" @default.
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