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- W2386735518 abstract "This study was designed to investigate the changes of prostaglandin I2 (PGI2) and nitric oxide (NO) secreted by endothelialized polyurethane small diameter artificial blood vessel. The peripheral blood mononuclear cells of healthy adult were separated and induced into endothelial progenitor cells (EPCs), which were identified by the methods of discrepancy microphage and fluorescent immunology labeling. After the induced cells being seeded on the polyurethane small-diameter artificial vessels, the endothelialized polyurethane small diameter artificial blood vessels were divided into four different experimental groups,including stationary group,low-flow shear stress group (5 dynes/cm2),medium-flow shear stress group (15 dynes/cm2), and high-flow shear stress group (25 dynes/cm2). Then, the levels of 6-keto-prostaglandin F1α(6-keto-PGF1α) and NO of different time were measured by enzyme-linked immunosorbent assay and nitrate reductase methods. The peripheral blood mononuclear cells differentiated into EPCs. They presented typical spindle-shaped appearance, and they were positively labeled by fluorescent acetylated-LDL,lectin,FLK-1 and vWF. Shear stress enhanced the production of NO and 6-keto-PGF1α by EPCs in a dose-dependent manner. Therefore, shear stress increases the secretion of NO and PGI2 by EPC, which suggests that shear stress can improve the antithrombogenic potentials of endothelialized polyurethane small diameter artificial blood vessel." @default.
- W2386735518 created "2016-06-24" @default.
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- W2386735518 date "2008-01-01" @default.
- W2386735518 modified "2023-09-26" @default.
- W2386735518 title "In Vitro Study of Regulation of Shear Stress on Antithrombogenic Potentials of Endothelialized Polyurethane Small Diameter Artificial Blood Vessel" @default.
- W2386735518 hasPublicationYear "2008" @default.
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