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- W2386737087 abstract "Objective Umbilical cord blood(UCB)is best containing a lot of endothelial progenitor cells (EPC)s,they are progenitors of vascular endothelial cells(VEC)sand capable of differentiation into VECs.It will lead to a new strategy to promote angiogenesis in bone tissue engineering.The purpose of this study was to an- alyze the potentiality on differentiation of mononuclear cells from rat umbilical cord blood into vascular endothelial cells in vitro.Methods Mononuclear cells were isolated from umbilical cord blood,and were cultured incondi- tioned medium(supplemented with 10%FBS,1%penicilin,1%phytomycin,VEGF 20μg/L,IGF-12μg/L, bFGF 2μg/L,EGF 20μg/L).The immunofluorescent staining was used to observe expressions and distributions of some special surface marker,such as CD34,CD133and vWF on adherent cells after 3 weeks and 6 weeks. Results The results of the immunofluorescent staining were positive on CD34,CD133 and vWF after 3 weeks, but their results were only positive on CD34 and vWF after 6 weeks,The part of adherent cells were positive onCD133,the positive cells were not found in the control group.Conclusion The mononuclear cells from rat um- bilical cord blood can be induced into endothelial progenitor cells after 3 weeks in vitro.The part of adherent cells may differentiate into vascular endothelial cells." @default.
- W2386737087 created "2016-06-24" @default.
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- W2386737087 date "2008-01-01" @default.
- W2386737087 modified "2023-09-25" @default.
- W2386737087 title "The Preparation and Identification of Vascular Endothelial Cells from Rat Umbilical Cord Blood" @default.
- W2386737087 hasPublicationYear "2008" @default.
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