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- W2386756089 abstract "As a highly sensitive nanomechanical sensor, microcantilever sensor is widely used in biochemical detection. Aflatoxin B1 (AFB1), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB1 are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB1with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB1 antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB1 concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB1, which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB1 dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB1 and may have great potential for food quality control and public health protection." @default.
- W2386756089 created "2016-06-24" @default.
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- W2386756089 date "2016-01-01" @default.
- W2386756089 modified "2023-09-25" @default.
- W2386756089 title "Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor" @default.
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- W2386756089 doi "https://doi.org/10.11648/j.ijmea.20160402.17" @default.
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