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- W2386765785 abstract "Objective:To investigate effects of expression of stem cell gene Nanog on esophageal cancer cell (ESCC) line TE-1 and on the sensitization to cisplatin. Methods:The expression of Nanog in ESCC cell line TE-1 was detected by immunofluorescence. The fluorescence labeled small interfering RNA (siRNA) was transfected into cells using Lipofectamine 2000. The transfection efficiency was observed by fluorescence microscopy. The silencing efficiency of siRNA was evaluated by RT-PCR and Western blot. Cisplatin was added 24 hours after transfection. After 48 h cultivation, the IC50 was measured in different transfection groups. The survival rates were detected in different transfection groups after adding cisplatin 5mg/L for 48 h. Results:The expression of Nanog protein was higher in ESCC cell line TE-1, and the expression was mainly in the nucleus. The cell transfection rate was (67.57±16.90)%. Silencing efficiency of Nanog siRNA was reached to 85% compared with that in negative control group. The value of IC50 was significantly decreased in Nanog siRNA group than that of negative control group and blank group. The cell survival rate was significantly decreased in Nanog siRNA group after treatment with 5 mg/L cisplatin for 48 h compared with that of negative control group and blank group. Conclusion:Nanog is highly expressed in ESCC cell line TE-1. Nanog siRNA efficiently reduces Nanog expression, which enhances cisplatin chemosensitivity in ESCC cell line." @default.
- W2386765785 created "2016-06-24" @default.
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- W2386765785 date "2012-01-01" @default.
- W2386765785 modified "2023-09-28" @default.
- W2386765785 title "The Effect of Silencing Nanog Gene Expression on Sensitization to Cisplatin in Esophageal Cancer Cell-Line" @default.
- W2386765785 hasPublicationYear "2012" @default.
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