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- W2386796365 abstract "Superoxide radicals(O 2 · ) produced in PSⅡ particles separated from spinach has been investigated by spin trapping electron spin resonance (ESR) technique. After bubbling with oxygen and incubating with tetracyanoethylene (TCNE) that acts as an inhibitor of superoxide dismutase (SOD) the experimental evidence of spin adduct of [DMPO O 2H] · can be obtained by in situ ESR measurements in PSⅡ particles. In contrast, adduct of O 2 · and DMPO produced under illumination is obviously decreased when SOD that usually performs as a scavenger of O 2 · is present. Furthermore, it is evident to find that the generation of O 2 · is positively correlated with the concentration of oxygen. The production of O 2 · is also pH dependent, and O 2 · concentration reaches maximum when pH is in the range of 6 0～6 5. Either high, or low pH value will lead it to descend steeply.In Tris HCl washed PSⅡ particles donor side ET inhibition can causes the production of O 2 · decreasing obviously. It can be concluded that the PSⅡ that is active in splitting water and oxygen generation is also a reactive site responsible for the photoinduced O 2 · generation in thylakoid of higher plant. In another word, the formation of O 2 · is relevantly correlated to the electron transport activity of PSⅡ." @default.
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- W2386796365 date "2001-01-01" @default.
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- W2386796365 title "ESR Studies on the Superoxide Radicals Generated in Photosystem II of Higher Plant" @default.
- W2386796365 hasPublicationYear "2001" @default.
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