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- W2386802912 abstract "[Objective] The qualitative and quantitative research on quercetin and kaempferol in Ardisia faberi Hemsl.were carried out to establish the HPLC analysis method,which provided a foundation for further study of pharmacodynamics of folk medicines-Ardisia faberi Hemsl..[Method] The main chemical compositions in different parts of Ardisia faberi Hemsl.were determined by using qualitative method.HPLC analysis was carried out based on Hypersil DJZ C18 chromatographic column(4.6 mm×200.0 mm,5 μm) and methanol-0.4% phosphoric acid(60∶40) as the mobile phase,the detection wavelength was 368 nm and the flow rate was 1.000 ml/min as well as the column temperature was 30 ℃.The number of theoretical plates was no less than 3 000 what calculated according to quercetin and kaempferol.[Results] The linear range of quercetin was among 0.081 6-0.489 6 μg(R2= 0.999 6),the average recovery was 101.6%,RSD=0.87%.The linear range of kaempferol was among 0.069 6-0.417 6 μg(R2=0.999 6),the average recovery was 96.8%,RSD=1.01%.The content of quercetin in the leafage was 0.038 2%,in the stem was 0.005 2%,in the whole plant was 0.010 5%.The content of kaempferol in the leafage was 0.027 6%,in the stem was 0.002 1%,in the whole plant was 0.009 6%.[Conclusion] HPLC analysis method was stable and the repeatability was better,quercetin and kaempferol consisted in different parts of plant.The content of the quercetin and kaempferol in the leafage was higher than the whole plant and the stem." @default.
- W2386802912 created "2016-06-24" @default.
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- W2386802912 date "2011-01-01" @default.
- W2386802912 modified "2023-09-24" @default.
- W2386802912 title "Qualitative and Quantitative Research on Quercetin and Kaempferol in Ardisia faberi Hemsl." @default.
- W2386802912 hasPublicationYear "2011" @default.
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