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- W2386840771 abstract "Different N~+ ion doses were implanted into an inosine-producing strain Bacillus subtilis HSD060 for the screening of anti-sulfaguanidine(SG~r) and arabinose utilization deficiency(ara) mutants.Two single mutants, B13(SG~r) and B67(ara~-),were obtained and their inosine yields in shake flask were increased by 16.3%and 20%, compared with the original strain.The comparatively higher mutation rate of SG~r mutants occurred at implantation dose of 3×10~(15) ions/cm~2,while that of ara~- mutants at 1×10~(16) ions/cm~2.Strains B13 and B67 were treated with the optimum N~+ ion dose once again for the screening of double mutated strains with SG~r and ara~-.Mutants B 13-105,B 13-5 and B67-1 were obtained with double mutations,the inosine yields of which in shake flask were increased by 23.5%,20.4%and 23.1%,respectively,compared with their parent strains." @default.
- W2386840771 created "2016-06-24" @default.
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- W2386840771 date "2009-01-01" @default.
- W2386840771 modified "2023-09-25" @default.
- W2386840771 title "Screening of High Inosine-producing Strains with Double Mutations of SG~r and ara~- by N~+ Ion Implantation" @default.
- W2386840771 hasPublicationYear "2009" @default.
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