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- W2386852248 abstract "To explore the best expressing condition of the recombinant 26-kilodalton glutathione S-transferases of S.japonicum(rSj26 GST) in Escherchia coli(E.coli) and to analyse its characterization.E.coli BL21(DE3) was transformed with plasmid pGEX-3X.The best expressing condition of rSj26 GST was explored through orthogonal design which took growth temperature,IPTG concentration,culture density,incubation time as indices.The expressed products(rSj26 GST) proteins were purified from supernatant bacterial lysates by affinity chromatography using reduced glutathione resin.It can be detected using functional assays and immunological methods.The pure rSj26 GST with the activity of glutathione S-transferase,and it could be recognized by the sera of rabbit and mouse infected with S.japonica.The rabbit was vaccinated with rSj26 GST and the highest level of the anti-rSj26 GST antibody was 1∶256 000.Western blot analysis showed that the anti-rSj26 GST antibody could recognize the antigen of adult worm of Schistosoma japonica.The best expressing condition of the recombinant 26-kilodalton glutathione S-transferases of S.japonicum(rSj26 GST) in E.coli was determined through orthogonal design." @default.
- W2386852248 created "2016-06-24" @default.
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- W2386852248 date "2006-01-01" @default.
- W2386852248 modified "2023-09-23" @default.
- W2386852248 title "Optimizing expression of recombinant 26-kilodalton glutathione S-transferases of Schistosoma japonicum in Escherchia coli" @default.
- W2386852248 hasPublicationYear "2006" @default.
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