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- W2386862921 abstract "Nicotinic acid biotransformation by immobilized Gibberella intermedia CA3-1 harboring nitrilase was investigated in this study. Chitosan-polyvinyl alcohol was found to exhibit great advantages over other composite materials,with contents of chitosan and polyvinyl alcohol 1.6% and 2%,respectively. The experimental results showed that the optimal transformation conditions were as follows:initial 3-cyanopyridine concentrations of 300mmol/L,transformation temperature of 30℃ and initial pH of 7.0. The substrate could be completely converted within 60 min. Immobilized cells could be reused for up to 26 batches as compared to 9 batches for free cells. And 283.5g nicotinic acid/(g dcw) was produced with 80.55% enzyme activity preserved. Its transformation capability was three times higher than that of free cells. In addition,the immobilization substantially enhanced its tolerance toward high 3-cyanopyridine concentration and improved its thermalstability. Immobilized cells could biosynthesize 191.3g nicotinic acid/(g dcw),retaining 30% enzyme activity by 19 fed-batches." @default.
- W2386862921 created "2016-06-24" @default.
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- W2386862921 date "2014-01-01" @default.
- W2386862921 modified "2023-09-24" @default.
- W2386862921 title "Biotransformation of 3-cyanopyridine for producing nicotinic acid by immobilized Gibberella intermedia" @default.
- W2386862921 hasPublicationYear "2014" @default.
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