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- W2386920830 abstract "Objective Study on the permeability of rhβ nerve growth factors (NGF) through blood brain barrier (BBB) to provide scientific basis for research of β NGF in clinical therapeutic application. Methods To transfect the COS 7 cell line with recombination expressive vector, PcDNA3 β NGF, by lipofectamine. The proteins, produced in the supernatant by the transfected cells, was isolated and purified by ion exchange chromatography on carboxymethyl cellulose. The biological activity of the purified protein was analyzed by the prospectation of processes grown out from PC12 cell line; and the protein was labelled with 125 I and injected i.v into rats, and then the radioactivity of rat brain tissue was analyzed by γ scintillometer. Results The purified protein could stimulate the process growth of PC12 cell line and the result of γ scintigraphy analysis showed that the radioactivity achieved high level in rat brain tissue 30 min after the protein injection. Conclusion Purified rhβ NGF has good biological activity and it could partially penetrate the BBB." @default.
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- W2386920830 date "2001-01-01" @default.
- W2386920830 modified "2023-09-26" @default.
- W2386920830 title "Study on permeability of the recombinant #beta#-NGF through blood-brain barrier" @default.
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