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- W2386981498 abstract "Objective To investigate the effect of Candida albicans and its components on the expression level of human beta-defensin-2 mRNA (HBD-2) in keratinocytes in vitro. Methods Different components of Candida albicans were isolated by lyticase, repeated freezing and thawing, sonication, and centrifugation. The keratinocytes and HaCaT cell lines were co-cultured with Candida albicans and its cellular components for 24 h. The expression level of HBD-2 mRNA was detected by reverse-transcription polymerase chain reaction (RT-PCR). Results Low expression level of HBD-2 mRNA in the unstimulated keratinocytes and HaCaT cells was detected. The HBD-2 mRNA expression levels in the keratinocytes stimulated by Candida albicans, the extract of its cell wall, and pure mannan were significantly increased (P 0.01, P 0.05, and P 0.01, respectively). No significant differences of the HBD-2 mRNA expression levels of the keratinocytes stimulated with the cultural supernatant and plasm extract of Candida albicans (P 0.05). Conclusions Candida albicans, the extract of cell wall of Candida albicans, and commercial mannan can increase the expression level of HBD-2 mRNA in keratinocytes." @default.
- W2386981498 created "2016-06-24" @default.
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- W2386981498 date "2005-01-01" @default.
- W2386981498 modified "2023-09-23" @default.
- W2386981498 title "The Effect of Candida albicans on the Expression Level of Human β-defensin-2 mRNA in Keratinocytes in vitro" @default.
- W2386981498 hasPublicationYear "2005" @default.
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