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- W2386998049 abstract "Objective To investigate the inhibitory effects of circular dum bb ell decoy oligod eoxynucleotides(ODNs )targeting NF-κB on the transcriptiona l activity of N F-κB binding its cis-elements and the ex pression of cytokine s(TNF-αand IL-6)in mesangial cells in vitro.Methods According toκB sequ ence,circular dumbbell decoy-ONDs including 58bases and 2copies ofκB seque nces were designed and synthesized.The inhibition effect of circular dumbbell d ecoy ODNs on th e transcriptional activity of NF-κB was detected by electropho retic mobilitory shift assay(EMSA).Mesangial cells extracted from rats were r andomly divided into normal c ontrol,LPS-stimulated,circular dumbbell decoy- ODNs treated,scram bled-decoy-ODNs treated and cation ic liposome treated gro up.Circular dumbbell decoy ODNs at the doses of 2mg /L,4mg /L and 8mg /L were transfected respectively into mesangial c ells from rats using cationic li poso me vector.Mesangial cell were stimulated with LPS at 6h after c ircular d umbbell decoy-ODNs transfection.Then,the transfected Supernatants and cells w ere collected at 8h,12h.Cytokines mRNA expressio n in mesangial cells was d etected by RT-PCR and cytokines protein level by ELISA.Results Circular dumbbe ll decoy ODNs could e ffectively inhibit the DNA binding a ctivity of NF-кB to its cis-elements in vitro.Circular dumbbell decoy ODNs at the doses of 4and 8mg /L could e ffectively inhibit the expression l evels of TNF-α,IL-6mRN A and protein in mesangial c ells.Conclusion Circular dumbbell decoy ODNs targe ting NF-κB could effectively inhibit the expr ession of cytokines in mesangial cells through the inhibition of NF-κB activity.[" @default.
- W2386998049 created "2016-06-24" @default.
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- W2386998049 date "2005-01-01" @default.
- W2386998049 modified "2023-09-28" @default.
- W2386998049 title "Inhibitory effect of circular dumbbell decoy ODNs targeting NF- кB on cytokinesexpression in mesangial cells in vitro" @default.
- W2386998049 hasPublicationYear "2005" @default.
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