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- W2387014808 abstract "Objective:To establish a procdure of culturing melanocytes in the absence of phorbol esters(TPA) and cholera toxin(CT).This method can avoid tumorigenesis of TPA and the toxicity of the CT. Methods:We used dispaseⅡ and trypsin to digest the skin tissue and obtain the melanocytes,and cultured the cells in medium which free of serum,phorbol esters and cholera toxin.The melanocytes were purified in the medium at first and then proliferated in the medium with serum.We observed the proliferation and cytodendrites of the melanocytes.Dopa and anti-100 antibody staining were used to identify the cell type.Results: After seven days of culture,the melanocytes were 70% confluence.They were dendritic cells with dipolar,tripolar or multipolar.After one or two passages,The cells can reach a purification of 98%.The results of dopa staining and anti-S100 immunocytochemistry were positive.Conclusion:This procdure can obtain the highly purified melanocytes which grew well in the keratinocyte serum free medium in the absence of phorbol esters and cholera toxin." @default.
- W2387014808 created "2016-06-24" @default.
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- W2387014808 date "2006-01-01" @default.
- W2387014808 modified "2023-09-23" @default.
- W2387014808 title "Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters and Cholera Toxin" @default.
- W2387014808 hasPublicationYear "2006" @default.
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