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- W2387019025 abstract "Objective To explore the effects of angiotensinⅡ(AngⅡ) on the expressions and transposition of protein kinase C(PKC) epsilon and alpha in cardiac fibroblasts,and to explore the role of these PKC subtypes in ventricles reconstitution.Methods The 2 to 4 generations of cardiac fibroblasts were treated with AngⅡof 10-6 mol/L(experimental group) or without AngⅡ(control group).The distribution and location of PKC epsilon and alpha were detected by indirect immunofluorescence assay.Fluorescence intensity was analyzed using the Image-pro-plus 4.0.Results Fluorescence microscopy showed that PKC epsilon resided in cell membrane,endochylema,and nucleo-cytoskeleton.Fluorescence intensity was obviously enhanced in the experimental group,especially in nucleo-cytoskeleton;PKC alpha resided mainly in nucleo-cytoskeleton in the control group,but it distributed in membrane,endochylema,and nucleo-cytoskeleton,with markedly enhanced fluorescence intensity.Half-quantitive intensity fluorescence of PKC epsilon and alpha was markedly higher in the experimental group than in the control group(P0.001).Conclusions AngiotensinⅡ has a marked effect on the expressions and transposition of PKC epsilon and alpha in cardiac fibroblasts,suggesting these two subtypes may play an important role in the signal transduction in cardiac fibroblasts." @default.
- W2387019025 created "2016-06-24" @default.
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- W2387019025 date "2010-01-01" @default.
- W2387019025 modified "2023-09-23" @default.
- W2387019025 title "Effects of angiotensin II on expressions and transposition of protein kinase C epsilon and alpha in cardiac fibroblasts" @default.
- W2387019025 hasPublicationYear "2010" @default.
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