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- W2387020143 abstract "Stone flounder(Kareius bicoloratus L.) is a marine fish with high economic value in the aquaculture industry of northern China.Continuous high-level mortalities occurred in stone flounder.The diseased samples were collected from wild stocks and cultured in farms during March 2001 and April 2002 in Qinhuangdao,Hebei Province.Examination of diseased stone flounder showed that the fish belonged to septicaemia infection.The biological characteristics of pathogenic bacteria were studied,including morphological characteristics,physiological and biochemical characteristics,G+C mol% of DNA,and plasmid content of isolates.In addition,the molecular identification of representative strains was conducted,and the 16S rRNA gene were partially sequenced and compared with sequences deposited in databases;molecular phylogenetic trees were constructed.The results showed that the isolates were Gram-negative,were arranged singly or in pairs,round-ends,medium-sized short rods,0.4-1.0?μm×1.0-2.0?μm in size,no-endospores,electron micrograp by negatively stained revealed that the cultures have no flagella;the physiological and biochemical traits showed that the isolates were positive reaction for oxidase,catalase,gelatin hydrolysis,maltose,D-mannitol,produce acid but no gas from glucose,reduce nitrate,tween 80,adonitol,D-raffinose,malonate utilization,and negative reaction for indole,methyl red,esculin,glycerol,urease,trehalose,sucrose reduction,arabinose,Voges-Proskauer reaction,produce H_(2)S,D-sorbitol,D-arabitol,i-erythritol,m-inositol,L-rhamnose,salicin,have no motility,can not growth at 37?℃,G+C mol% of DNA were 62.6%;the sequenced 16S rRNA gene of strain HQ010320-1(GenBank accession no.AY786177) is 1?425?bp in length,and the sequenced 16S rRNA gene of strain HQ010320-5(GenBank accession no.AY786178) is 1?423?bp in length.The 16S rRNA gene sequence of representative strains(HQ010320-1 and HQ010320-5) exhibited high similarity(99% and 100%) with the 16S rRNA gene of A.salmonicida from GenBank database.The results showed that the identified 60 strains belonged to new subspecies of Aeromonas salmonicida,and named as Aeromonas salmonicida subsp.flounderacida subsp.nov.by their morphological characteristics,physiological and biochemical characteristics,and molecular identification by 16S rRNA partial sequence.[Journal of Fishery Sciences of China,2006,13(6):917-923]" @default.
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- W2387020143 date "2006-12-04" @default.
- W2387020143 modified "2023-09-28" @default.
- W2387020143 title "Biological characterization and phylogenetic analysis of pathogenic new subspecies of Aeromonas salmonicida isolated from stone flounder(Kareius bicoloratus L.)" @default.
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