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- W2387035281 abstract "For cloning the cytokine human Midkine (MK) gene, we designed a pair of PCR specific primers according to the reported human MK cDNA sequence in GenBank. The target DNA fragment was obtained by RT-PCR from gastric carcinoma patient's carcinogenesis tissue and cloned into pMD18 T-vector. After sequencing the MK nucleotide fragment was inserted into an E.coli expression vector pET30(a+).The recombinant plasmid (30 MK) was obtained and transferred into E.coli BL21 (DE3), the resulted E.coli pEMK was cultured and induced with IPTG, the efficiently expressed recombinant MK protein was purified from the total cellular soluble protein by Heparin Sepharose 4B column. Moreover, MTT methods was used and determined that the recombinant MK protein possessed the activity to promote the growth of NIH3T3 cells." @default.
- W2387035281 created "2016-06-24" @default.
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- W2387035281 date "2005-01-01" @default.
- W2387035281 modified "2023-09-22" @default.
- W2387035281 title "Cloning and expression of Midkine cytokine from carcinogenesis tissue in E.coli" @default.
- W2387035281 hasPublicationYear "2005" @default.
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