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- W2387062685 abstract "Identification of marine microalgae using a combination of both morphology and molecular sequences has been more and more recognized to be the future direction.The prerequisite to do so is to establish pure cell culture and to obtain first-hand information of proper culture conditions including all the environmental parameters.In this investigation,we present our data on classifying three isolates from the coastal water through both molecular and morphological analyses.Analyses of the 18S ribosomal RNA gene(18S rDNA) and internal transcribed spacer(ITS) sequences showed that they are Symbiodinium sp.,Scrippsiella trochoidea and Heterosigma akashiwo.To understand their growth characteristics,different culture conditions’ effects on growth were monitored,using A680as an estimation of biomass.The results showed that Symbiodinium sp.,S.trochoidea and H.akashiwo all gave the best growth performance at 22°C,reaching 9.28×105,7.9×104 and 4.75×105cells/mL respectively in batch cultures.Both elevated irradiance and longer photoperiods benefited the growth.However,S.trochoidea entered recession phase earlier under higher irradiance level.All three species were shown to be able to tolerate a wide range of sainities(15 to 36).Concentrations of 882 μmol/L of 3 NO and 36.2 μmol/L of 3 4 PO in the medium were proved to be sufficient for fast accumulation of algal biomass." @default.
- W2387062685 created "2016-06-24" @default.
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- W2387062685 date "2012-01-01" @default.
- W2387062685 modified "2023-09-26" @default.
- W2387062685 title "Molecular identification and culture trials of three marine microalgae" @default.
- W2387062685 hasPublicationYear "2012" @default.
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