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- W2387078128 abstract "Objective To construct a Pichia pastoris expression plasmid pPIC9K-GRA1,express dense granules antigen-1(GRA1) of Toxoplasma gondii,and to lay a foundation for further research the function of GRA1 protein.Methods The GRA1 opening reading frame(ORF) was amplified by PCR.The ORF and plasmid pPIC9K were digested with EcoRⅠ and NotⅠ respectively,then ligated into the pPIC9K at polylinker.The recombinant vector pPIC9K-GRA1 was characterized by PCR,restriction enzyme digestion,and sequencing analysis.Results The expected fragement was amplified by PCR and digested by double enzyme.The sequence results showed that the recombinant plasmid pPIC9K-GRA1 contained GRA1 ORF with the right orientation.Conclusion The pichia pastoris expression vector pPIC9K-GRA1 is successfully constructed." @default.
- W2387078128 created "2016-06-24" @default.
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- W2387078128 date "2007-01-01" @default.
- W2387078128 modified "2023-09-28" @default.
- W2387078128 title "Construction and identification of recombinant plasmid pPIC9K-GRA1 from Toxoplasma gondii" @default.
- W2387078128 hasPublicationYear "2007" @default.
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