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- W2387078356 abstract "The effects of natto strain BNK cultivation time,operational times,temperature and concentration of lysozyme on protoplast production and regeneration were studied.In addition,different operational methods, regeneration media,osmotic stabilizers and protective additives were also investigated.The appropriate conditions of cultur are 9h,BNK treated by 0.8 mg/ml lysozyme for 40 min at 30℃,then spreaded on PYG medium with mannitol as stabilizer and starch as a protector.Under these conditions the protoplast formation achieves 98.2% and regeneration frequency 28.4%." @default.
- W2387078356 created "2016-06-24" @default.
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- W2387078356 date "2007-01-01" @default.
- W2387078356 modified "2023-09-25" @default.
- W2387078356 title "Study on Conditions for Protoplast Production and Regeneration of Bacillus subtilis natto" @default.
- W2387078356 hasPublicationYear "2007" @default.
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