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- W2387085918 abstract "Objective To study whether adult bone marrow stromal cells(BMSCs) could be induced to differentiate into neural stem cells (NSCs) and neurons by basic fibroblast growth factor(bFGF) and epidermal growth factor(EGF). Methods(Adult marrow stromal) cells were cultured from bone marrow of four-week-old Wistar rats.bFGF and EGF were used to induce the thirdgeneration BMSCs,and contrasted with the cells that had not been induced.A phase contrast microscope was used to observe the differentiation of BMSCs,daily.Immunohistochemistry technique was used to identify the differentiated BMSCs with Nestin,neuron specific enolase(NSE) and glial fibrillary acid protein(GFAP). Results After seven days of culture,some BMSCs appeared typical morphological neuronal traits: the refractile and cone-like or spherical cell bodies and long processes,expressed Nestin or NSE or GFAP.The three kinds of cells showed significant difference,but the cells in the control group still had no Nestin or NSE or GFAP expression. Conclusion bFGF and EGF could induce BMSCs to differentiate into NSCs and neurons,and might regulate the differentiation and promote the maturation of neurons." @default.
- W2387085918 created "2016-06-24" @default.
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- W2387085918 date "2006-01-01" @default.
- W2387085918 modified "2023-09-25" @default.
- W2387085918 title "BMSCs DIFFERENTIATION INTO NSCs INDUCED BY bFGF AND EGF" @default.
- W2387085918 hasPublicationYear "2006" @default.
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