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- W2387092232 abstract "Objective:To establish a method of loop-mediated isothermal amplification for rapidly detecting Streptococcus pneumoniae in Sputum samples.Methods:Four S.pneumoniae-specific LAMP primers which be aimed at the gene of autolysin(lytA) were designed from the published sequence of strain R6.then detected Streptococcus pneumoniae in sputum samples by LAMP method and detected by traditional and PCR methods at the same time.Results:A total of 54 samples were detected in our experiment,the traditional,PCR and LAMP method have 27,31 and 36 positive test results respectively.The analysis showed that there was no statistically significant difference between the three methods(P0.1).Conclusion:In spite of the three methods in our experiment have no significant differences,the LAMP technique is more rapid and convenient compared with PCR method and traditional culture method,so this method is still very useful for the rapid detection of S.pneumoniae." @default.
- W2387092232 created "2016-06-24" @default.
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- W2387092232 date "2009-01-01" @default.
- W2387092232 modified "2023-09-27" @default.
- W2387092232 title "Detection of Streptococcus pneumoniae in sputum samples by loop-mediated isothermal amplification method" @default.
- W2387092232 hasPublicationYear "2009" @default.
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