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- W2387129905 abstract "The copper-regulated gene expression system has been developed to control spacial and temporal expression of transgene in plant It comprises two parts: (1) ace1 gene encoding copper-responsive transcription factor under the control of a constitutive or organ-specific promoter, and (2) a gene of interest under the control of a chimeric promoter consisting of the CaMV 35S (-90 to +8) promoter linked to the metal responsive element (MRE) carrying activating copper-metallothionein expression (ACE1)-binding sites. Here, the effectiveness of two different AGE1-binding cis -elements which derive from 5'-regulatory region of yeast metallothionein gene was investigated in transgenic tobacco (Nicotiana tabacum L cv. W38). The results revealed that the MRE (-210 to -126) could increase the system inducibility by 50% - 100% compared with the previously reported MRE (-148 to -105). It is potential to use the copper-inducible system to control valuable gene traits in plant biotechnology." @default.
- W2387129905 created "2016-06-24" @default.
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- W2387129905 date "2003-01-01" @default.
- W2387129905 modified "2023-10-12" @default.
- W2387129905 title "Improvement of Copper-inducible Gene Expression System for Plant" @default.
- W2387129905 hasPublicationYear "2003" @default.
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