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- W2387131752 abstract "Objective To evaluate recombinant adeno associated virus (AAV) as an in vivo gene transfer vector for the retina and to explore the possibility of monitoring the expression of green fluorescent protein (GFP) using a flat mounting method and a noninvasive method. Method rAAV gfp were prepared and purified into 10×10 12 TU·L -1 . Chinchilla rabbits and Japanese white rabbits were injected subretinally with 10μL rAAV gfp. Four Chinchilla rabbits were killed severally at 3, 5, 6 and 10 weeks and the eyes were enucleated. The eyecups were cut peripherally in four or five places and flat mounted on a glass slide for fluorescence microscope observation. Three Japanese white rabbits were injected with rAAV gfp for longitudinal studied and the fluorescence was monitored by once a week retinal fluorescence photography. Result The signal was detected after 3 weeks. Gradual fluorescence increase in the number of transduced cells occurred from 3 weeks to 10 weeks. The fluorescence area was larger and the intensity was stronger with time changing. The GFP signal was detected in all the animals as early as 4 weeks. A gradual increase in the number of transduced cells occurred throughout the experimental period of 7 months. Conclusion The subretinal injection has good transduction effect. It is possible to make stable expression of rAAV gfp transduced in the retina. As a marker gene, gfp is suitable for retina AAV transduction." @default.
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- W2387131752 date "2001-01-01" @default.
- W2387131752 modified "2023-09-23" @default.
- W2387131752 title "Experimental study on adeno-associated virus -mediated genetransfer into the rabbit retina" @default.
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