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- W2387134062 abstract "The coding sequence of Morus bombycis ubiquitin gene is cloned and determined.The opening reading frame(ORF) is 240 bp in length,encoding 79 amino acids with the molecular weight of 8.74 kD and theoretical isoelectric point of 7.16.The theoretical three-dimensional structure of this gene is displayed by homology modeling.Using pET-32a(+) as a fused expression vector,a recombinant plasmid pET-32a-ub containing the coding sequence of M.bombycis ubiquitin gene is constructed.Western blot indicates that the M.bombycis ubiquitin gene is expressed successfully in the BL21(DE3) strain of E.coli induced with IPTG.The results may serve as basis for further study on the function of ubiquitin gene in M.bombycis." @default.
- W2387134062 created "2016-06-24" @default.
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- W2387134062 date "2009-01-01" @default.
- W2387134062 modified "2023-09-24" @default.
- W2387134062 title "Cloning,Sequence Analysis and Prokaryotic Expression of the Ubiquitin Gene of Morus bombycis" @default.
- W2387134062 hasPublicationYear "2009" @default.
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