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- W2387173882 abstract "To explore the effects of recombinant vector Stella on the pluripotency of mouse embryonic stem cells( ESCs)and expression profile of specific meiosis marker-Stra8 in transfected ESCs. The eukaryotic expression vector containing Stella was constructed and transfected into ESCs. Expression profiles of GFP was observed in transfected ESCs,and expression levels of pluripotent genes and specific meiosis markers were simultaneously detected by RT-PCR and immunofluorescent staining. The results demonstrated that although Stella which had been cloned in the recombinant vector was successfully expressed in ESCs as identified by GFP and PCR amplification,it had no significant effect on the expression profiles of pluripotent and specific meiosis marker genes." @default.
- W2387173882 created "2016-06-24" @default.
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- W2387173882 date "2014-01-01" @default.
- W2387173882 modified "2023-09-23" @default.
- W2387173882 title "Construction of Stella Eukaryotic Expression Vector and Its Effect on Pluripotency of Mouse Embryonic Stem Cells" @default.
- W2387173882 hasPublicationYear "2014" @default.
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