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- W2387177741 abstract "AIM:To establish an in vitro root canal model for analyzing bacterial endodontic microleakage.METHODS:The root was mounted into an Eppendorf tube and the orifice was left opened in the Eppendorf tube (coronal chamber).The contacting area between the root surface and the Eppendorf tube was sealed,and the apical end of the root was placed in a glass bottle containing sterile Brain Heart Infusion (BHI).The coronal chamber was filled with 0.1 mL bacterial inoculum and the apical glass bottle was filled with 1 mL fresh BHI.Fresh BHI was added into the coronal chamber in a 3-day-interval and the turbidness in the apical glass bottle was observed daily for 90 days. Eighteen extracted single-root human anterior teeth were collected and divided randomly into three groups:the gutta-percha obturation group (6 roots) in which the canals were obturated using cold gutta-percha and lateral condensation technique;the vacant-canal group (6 roots) in which the canals were instrumented and left un-obtuarted; the hermetic-canal group (6 roots) in which the canals were obturated and the surfaces of the root (included the canal orifice and the apical foramen) were completely sealed.RESULTS:All vacant-canal canals showed bacterial penetration within 24 h,while the hermetic-canal canals remained uncontaminated throughout the 90 days of the test period.Two canals in the test group were found having bacterial leakage at the 44th and 46th day,respectively.CONCLUSION:The in vitro model designed is practicable and effective,it has, to some extent,the clinical relevance and feasibility." @default.
- W2387177741 created "2016-06-24" @default.
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- W2387177741 date "2006-01-01" @default.
- W2387177741 modified "2023-09-23" @default.
- W2387177741 title "Establishment of an in vitro root canal model for evaluating bacterial endodontic leakage" @default.
- W2387177741 hasPublicationYear "2006" @default.
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