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- W2387197330 abstract "To express and immunoidentify goose tembusu virus envelope domain Ⅱ in Escherichia coli,the encoding gene of goose tembusu virus JS804 strain envelope protein domain Ⅱ was artificially synthesized with the length of 507 bp.The synthesized gene was then inserted into the prokaryotic vector pGEX-4t-1 for the construction of recombinant expression plasmid pGEX-EII and then transformed into E. coli BL21( DE3). The recombinant protein with the molecular mass of4. 4 × 104 was successfully expressed in E. coli by optimizing the conditions of expression. Western-blot and IFA revealed the good immunogenicity exhibited by the recombinant protein." @default.
- W2387197330 created "2016-06-24" @default.
- W2387197330 creator A5050764353 @default.
- W2387197330 date "2015-01-01" @default.
- W2387197330 modified "2023-09-26" @default.
- W2387197330 title "Expression and immunology of domain II of goose tembusu virus envelope protein in Escherichia coli" @default.
- W2387197330 hasPublicationYear "2015" @default.
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