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- W2387220639 abstract "Ammonia-oxidizing archaea are likely the most abundant ammonia-oxidizing microbes in natural environment and they also play an important role in nitrification.In order to improve nitrogen use efficiency and explicate the indicating function of ammonia-oxidizing archaea(AOA) on changes of soil quality in the Loess Plateau,AOA community structure diversity was studied.The soil samples used in this research derived from Changwu Agro-ecological Experimental Station on the Loess Plateau,Chinese Academy of Sciences,which had received 23 years continuous fertilization treatments,include CK(control,without fertilizers),LD(unplanted,without fertilizers),N(nitrogen input),P(phosphorus input) and NP(combination of nitrogen and phosphorus fertilizers).The soil AOA community structure diversity was analyzed by restriction fragment length polymorphism(PCR-RFLP) and DNA sequence.Positive clones collected randomly from clone libraries were digested by Rsa I and Msp I,respectively.According to the statistics of diversity index,there were 25,18,29,20 and 30 restriction endonuclease types(OTUs),respectively.The α diversity indices indicated that there was a pronounced difference among five fertilizer treatments.The OTUs were the highest in both P treatment and CK treatment,while the lowest in NP treatment.The rescaled distance matrix tree indicated that the different fertilization had weak convergence of AOA community types with the CK treatment soil.Phylogenetic tree of amoA gene amino acid sequences analysis showed all AOA sequences fell within cluster S and cluster M,but the proportions were different.These results indicated that long-term fertilization resulted in change of AOA community diversity;however,different fertilizer alkaline soil had no significant impact on the species composition of dominant AOA." @default.
- W2387220639 created "2016-06-24" @default.
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- W2387220639 date "2012-01-01" @default.
- W2387220639 modified "2023-09-25" @default.
- W2387220639 title "Effects of Long-term Nitrogen and Phosphate Fertilization on Diversity of Ammonia-Oxidizing Archaea in Dry Highland Soil of Loess Plateau,China" @default.
- W2387220639 hasPublicationYear "2012" @default.
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