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- W2387231626 abstract "Objective To clone the human thioredoxin gene(hTRX) and to construct the recombinant adenovirus vector containing hTRX. Methods The full length of TRX cDNA,which was obtained from recombinant plasmid pGEM-T Easy/TRX by restriction enzyme digestion,was inserted into pshuttle2 vector to form pshuttle2-TRX recombinant plasmid.Then pshuttle2-TRX was double digested to get the target fragment with CMV promoter which was inserted into DNA of Adeno-X virus induced by Tet-Off.The new plasmid was identified by PCR and restriction enzyme digestion. Results The pGEM-T Easy/TRX recombinant plasmid was sequenced,and the sequence contained the full length of TRXc DNA,which was in accordance with GeneBank sequence J04026.Pshuttle2-TRX shuttle vector containing TRX was constructed.The pAdCMV-TRX recombinant adenovirus vector by Tet-Off induction was gained successfully. Conclusions The successful cloning of TRX gene and construction of its adenovirus vector pAdCMV-TRX provide a new method for further studies concerning the bioactivity of TRX and the diseases closely related to oxidation damage,such as viral myocarditis,arteriosclerosis,cardiomyopathy,diabetes mellitus and Alzheimer's disease." @default.
- W2387231626 created "2016-06-24" @default.
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- W2387231626 date "2009-01-01" @default.
- W2387231626 modified "2023-09-24" @default.
- W2387231626 title "Construction of recombinant adenovirus vector with TRX gene by Tet-Off induction" @default.
- W2387231626 hasPublicationYear "2009" @default.
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