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- W2387252801 abstract "Objective To investigate the expression changes of translation elongation factor 1α1 in anti-BPDE transformed and carcinoma 16 HBEs. Methods Suppression subtractive hybridization (SSH), bioinformatics and semi-quantitative RT-PCR were applied. The cDNA of anti-BPDE transformed and carcinoma 16 HBE cells were used as tester respectively, and the cDNA of normal 16 HBE was used as driver, the library of subtractive hybridization were profiled and inverted into TA cloning vector after two times of hybridization and two times of PCR. After the screening, sequencing and analysis of sequences, semi-quantitative RT-PCR was performed accompanying to the inner reference of β-actin. Results 9 differentially expressed fragments were consistent with translation elongation factor 1α1 in different regions in Genbank, and the expressions were up regulated in BPDE transformed and carcinoma 16 HBE cells. Conclusion Translation elongation factor 1α1 may be related to the transforming effect and carcinogenesis of anti-BPDE." @default.
- W2387252801 created "2016-06-24" @default.
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- W2387252801 date "2005-01-01" @default.
- W2387252801 modified "2023-09-28" @default.
- W2387252801 title "Expression of Translation Elongation Factor 1α1 in 16 HBE Cells Induced by Anti-Benzo(a)pyrene-7, 8-diol-9, 10-epoxide" @default.
- W2387252801 hasPublicationYear "2005" @default.
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