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- W2387288975 abstract "A real-time PCR approach was used to study inhibition effects of the silicon surface on amplification of the hepatitis B virus genome. H-terminated silicon and natively oxidized silicon were tested in PCR reagents (with Taq DNA polymerase, but without DNA in) with deliberate designs. After slight oscillations, supernatants were extracted and mixed with DNA to perform the real-time amplification and detection. Fluorescence histories indicated that:(1) Compared to the H-terminated silicon, the samples with a native oxide layer restrained the polymerase catalytic activity much more severely, and the 1 mm2 native oxide surface could restrain at least 4.4 mU polymerases’ catalytic activity;(2)The amplification processes could not even realize an exponential form at the beginning of the amplification for these high surface-to-volume ratio cases;(3) Longer mixing time of silicon pieces and PCR reagents resulted in lower amplification efficiency." @default.
- W2387288975 created "2016-06-24" @default.
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- W2387288975 date "2008-01-01" @default.
- W2387288975 modified "2023-09-23" @default.
- W2387288975 title "Experimental Studies of Silicon Inhibition Effects on Polymerase Chain Reaction:A Real-Time Approach" @default.
- W2387288975 hasPublicationYear "2008" @default.
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