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- W2387299168 abstract "The objective of the present study was to compare effects of different culture systems on culture of bovine fetus fibroblasts in vitro, and the feasibility of culturing fibroblast with bovine serum albumin(BSA) instead of fetal calf serum(FCS). The fibroblasts were cultured with M199, DMEM, α-MEM and DMEM /F12, respectively, and the results indicated that α-MEM was more favorable for fibroblasts culture than other medium. Then bovine fetus fibroblasts were cultured in α-MEM mediums with 2, 4, 6, 8, and 10 mg/mL BSA, respectively. In primary culture, the effects of five levels BSA did not influence the cells that began to grow. Fibroblasts and epithelia had grown mixedly out tissues after culturing 48 h. But in subculturing, fibroblast cells cultured in α-MEM with 8 mg/mL BSA obtained higher attachment rate than that cultured with other mediums(P 0.05). The results indicated that BSA could substitute FCS for culturing bovine fetus fibroblasts, and the culture system preferably for fibroblasts was α-MEM mediums with 8 mg/mL BSA." @default.
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- W2387299168 date "2007-01-01" @default.
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- W2387299168 title "Effects of Different Culture Systems on Culture of Bovine Fetus Fibroblast Cells in vitro" @default.
- W2387299168 hasPublicationYear "2007" @default.
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