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- W2387305439 abstract "The effect of pressure on the catalytic properties of glutamate racemase from Aquifex pyrophilus, an extremophilic bacterium, was investigated. The activation volume for the overall reaction (ΔV ¬= ) and catalysis (ΔV ¬= cat ) was -96.97 ml/ mol and 4.97 ml/mol, respectively, while the reaction volume for the substrate binding (ΔV Km -1) was -101.94 ml/mol. The large negative ΔV ¬= for the overall reaction indicated that the pressurization of glutamate racemase resulted in enhanced catalytic efficiencies. In addition, this value was also due to the large negative ΔV Km -1 for the substrate binding. The negative value of ΔV Km -1 implied that the conformational changes in the enzyme molecule occurred during the substrate binding process, thereby increasing the degree of hydration. The small value of ΔV ¬= cat suggested that the pressure did not affect the glutamate racemase catalysis after the substrate binding." @default.
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- W2387305439 date "2002-01-01" @default.
- W2387305439 modified "2023-09-24" @default.
- W2387305439 title "Effect of pressure on catalytic properties of glutamate racemase from Aquifex pyrophilus, an extremophilic bacteria" @default.
- W2387305439 hasPublicationYear "2002" @default.
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