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- W2387328906 abstract "Objective:To establish a simple,rapid,sensitive column-switching HPLC method for the analysis of the 10-hydroxycamptothecin(HCPT) in human serum.Methods: A precolumn containing restricted access media(RAM) was used for the sample clean-up and trace enrichment and was combined with a C18 column for the final separation.The HCPT was monitored with fluorescence detector,excitation and emission wavelengths being 385 and 539 nm,respectively.Results: The analytical time was 8 minutes.There was a linear response range of 1~1 000 ng/mL with correlation coefficient of 0.998 while the limit of detection was 0.1 ng/mL.The intraday and interday variations were less than 5%.Conclusion: This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model were calculated." @default.
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- W2387328906 date "2007-01-01" @default.
- W2387328906 modified "2023-09-25" @default.
- W2387328906 title "HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study" @default.
- W2387328906 hasPublicationYear "2007" @default.
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