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- W2387331091 abstract "Objective To evaluate the application value of multiparameter flow cytometry(FCM) in screening minimal residual disease(MRD) in children with B lineage acute lymphoblastic leukemia(B-ALL).Methods On the basis of the leukemia-associated immunophenotypes(LAIPs) of 53 children with B-ALL,the MRD of 68 consecutive bone marrow specimens from 53 patients who achieved complete remission were detected by 4-color antibody combination,mainly CD19-APC/CD45-PerCP/CD10-FITC/CD34-PE,CD45/SSC and CD45/CD19 gating FCM.Results Eighteen LAIPs were detected in 53 cases with B-ALL.Abnormal expression of CD45,CD10,CD19,and CD34 were detected in 98.11% of the 53 patients.12 patients were MRD positive and had 0.012%～0.55% of residual leukemia cells.7 patients(58.3%) had the modulation of LAIPs,the LAIPs of 10 cases were monitored by the antigen panel of CD19/CD45/CD10/CD34.Conclusion According to the LAIPs of children with B-ALL,multiparameter FCM using 4-color antibody panel,including mainly CD19/CD45/CD10/CD34,is appropriate to detect MRD." @default.
- W2387331091 created "2016-06-24" @default.
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- W2387331091 date "2012-01-01" @default.
- W2387331091 modified "2023-10-11" @default.
- W2387331091 title "Detection of minimal residual disease in children with B lineage acute lymphoblastic leukemia by multiparameter flow cytometry" @default.
- W2387331091 hasPublicationYear "2012" @default.
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