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- W2387333982 abstract "Objective:To investigate the effect of dexamethasone expressing level of HSP90 induced by prolactin in Jurkat cells.Methods:The expression of HSP90 in Jurkat cells was analyzed at mRNA and protein levels by either revese-transcription polymerase chain reaction(RT-PCR) or Westetn blot assays following incubation with each of four concentrations(25 ng/ml,50 ng/ml,100 ng/ml and 200 ng/ml)of prolactin.After cultured with dexamethasone at different concentrations(10-9-10-6 mol/L),Jurkat cells were incubated with prolatin(final concentration of 100 ng/ml),and the expression of HSP90 was analyzed.Results:All the groups cocultured with prolactin at different concentrations were shown higher levels of HSP90(P0.05)compared with the group in the absence of prolactin.The levels of HSP90 were depended on the concentrations of prolactin untill it reached 100 ng/ml.The groups cultured with both dexamethasone and prolactin were shown lower levels of HSP90(P0.05) compared with the blank control group.Conclusion:Prolactin can increase the expression of HSP90 in human leukemic T cell line(Jurkat cells),and the maximal effection was shown on the concentration above 100 ng/ml of rhPRL.Dexamethasone can inhibit the expression of HSP90 in Jurkat cells stimulated by rhPRL." @default.
- W2387333982 created "2016-06-24" @default.
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- W2387333982 date "2008-01-01" @default.
- W2387333982 modified "2023-09-25" @default.
- W2387333982 title "Effect of dexamethasone on expression of HSP90 induced by prolactin in Jurkat cells" @default.
- W2387333982 hasPublicationYear "2008" @default.
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