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- W2387351633 abstract "Aim To explore the primary culture of isolated rat cerebral cortex neuron and the stress characteristics of cortical neurons endoplasmic reticulum induced by thapsigargin.Methods The cortical neurons of SD suckling mouse cultured in vitro,were indentified by immunohistochemical and immunofluorescence stain,the morphological change of apoptotic nucleus and measure cell apoptosis rate,the morphological change of apoptotic nucleus and measure cell apoptosis rate,were observed by Hoechst 33 258 simple staining and the the neurons subcellular structure was observed under a transmission electron microscope.Results The cortical neurons of SD suckling mcie was purified and cultured in vitro,the neurons were stained with NSE immunofluorescence stain and the total NSE positive cells 7 days after culture accounted for(95 ± 3)%.The apoptosis rate of in vitro primary culture neurons,in the 2μmol TG for duration of 24h was the highest of 19%± 0.54(P 0.05) with typical apoptosis morphological changes in 2μmol/lTG for a duration of 24h in addition to the nebulous structure of perikaryon endoplasmic reticulum,mitochondria and the membane breakage,enriched chromatin,karyopyknosis,cells shrink,nucleus splintered as well.Conclusion The modified culture in vitro allow to obtain higher purified neurons.The morphological changes of mitochondria may be due to downstream events at stress of cortical neurons endoplasmic reticulum induced by thapsigargin." @default.
- W2387351633 created "2016-06-24" @default.
- W2387351633 creator A5088464262 @default.
- W2387351633 date "2011-01-01" @default.
- W2387351633 modified "2023-09-23" @default.
- W2387351633 title "Primary culture of isolated rat cerebral cortex neuron and characteristics of neuron after interference with thapsigargin" @default.
- W2387351633 hasPublicationYear "2011" @default.
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